By Koniarova, I. and Ledecky, V. and Orsag, A., Veterinarni Medicina, 1993
Description
Altogether 52 tissue samples and swabs from the corium of the claw of diseased cattle were investigated. Microbiological examinations revealed the presence of 11 strictly anaerobic bacterial strains that belonged to the species Peptococcus asacharolyticus, Pc. sacharolyticus, Peptostreptococus anaerobius, Bacteriodes asacharolyticus and B. melaninogenicus. The remaining isolates were classified as Bacteroides, Fusobacterium, Streptococcus and Clostridium. In the above mentioned strains observations of their biochemical, morphological and enzymatic properties (Tab. I) were carried out. Their resistance to antibiotics was evaluated from the qualitative viewpoint (Tab. II). Capillary isotachophoresis was used to determine the amounts of volatile fatty acids that developed during glucose fermentation (Tab. III). In the single clinical samples the above-mentioned strains occurred as association of microorganism. The anaerobic genera Bacteroides and Fusobacterium were found in 52 % of the samples (Bacteriodes sp. and Fusobacterium sp. were recorded in 25 % and 12 % of the samples, respectively, whereas in 15 % of the samples both species occurred). According to our results the etiology of dermatitis digitalis et interdigitalis is closely connected with the incidence of anaerobes. Of the latter, those belonging to the genera Bacteroides and Fusobacterium are of major importance
Altogether 52 tissue samples and swabs from the corium of the claw of diseased cattle were investigated. Microbiological examinations revealed the presence of 11 strictly anaerobic bacterial strains that belonged to the species Peptococcus asacharolyticus, Pc. sacharolyticus, Peptostreptococus anaerobius, Bacteriodes asacharolyticus and B. melaninogenicus. The remaining isolates were classified as Bacteroides, Fusobacterium, Streptococcus and Clostridium. In the above mentioned strains observations of their biochemical, morphological and enzymatic properties (Tab. I) were carried out. Their resistance to antibiotics was evaluated from the qualitative viewpoint (Tab. II). Capillary isotachophoresis was used to determine the amounts of volatile fatty acids that developed during glucose fermentation (Tab. III). In the single clinical samples the above-mentioned strains occurred as association of microorganism. The anaerobic genera Bacteroides and Fusobacterium were found in 52 % of the samples (Bacteriodes sp. and Fusobacterium sp. were recorded in 25 % and 12 % of the samples, respectively, whereas in 15 % of the samples both species occurred). According to our results the etiology of dermatitis digitalis et interdigitalis is closely connected with the incidence of anaerobes. Of the latter, those belonging to the genera Bacteroides and Fusobacterium are of major importance
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